Re: [ccp4bb] Color of heme containing Xtals

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From: byron delabarre byron {- dot -} ccp4 {- at -} GMAIL {- dot -} COM
Date: 2008-03-14

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Date: 2008-03-14

Subject: Re: Color of heme containing Xtals
From: Dominique BOURGEOIS dominique {- dot -} bourgeois {- at -} IBS {- dot -} FR
Date: 2008-03-14

Hello,

A more precise view on the spectroscopic signature of your crystalline
heme protein can be obtained by microspectrophotometry (UV-vis
absorption, Raman or fluorescence). For such studies, people are
welcome to apply for using the Cryobench lab at ESRF
(http://www.esrf.eu/UsersAndScience/Experiments/MX/Cryobench/). A
number of microspec are also lying around or can be purchased (for
example: http://www.4dx.se/spectrop.htm).

Dominique.

Jan Schoepe a écrit :

type="cite">Hello everybody,

I wonder if anybody has experience with heme (or to be more precise:
heme b) containing proteins which Xtals do not look red under the
microscope. How might the technique for crystallization (e.g. sitting
drop, hanging drop) influence the intensity of the color? Many thanks!

Jan

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CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 14 March 2008

Previous message:
Subject: trim back to Cbeta in coot : summary
From: byron delabarre byron {- dot -} ccp4 {- at -} GMAIL {- dot -} COM
Date: 2008-03-14

Next message:
Subject: energy minimise ligand model
From: Thomas Edwards T {- dot -} A {- dot -} Edwards {- at -} LEEDS {- dot -} AC {- dot -} UK
Date: 2008-03-14