[ccp4bb] Tricks to solubilize protein

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 14 March 2008

Previous message:
Subject: Re: exclude range within data in scala , discontinuous run in scala problem
From: hari jayaram harijay {- at -} GMAIL {- dot -} COM
Date: 2008-03-14

Next message:
Subject: Re: exclude range within data in scala , discontinuous run in scala problem
From: Phil Evans pre {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-03-15

Subject: Tricks to solubilize protein
From: Ju He julie {- dot -} he {- at -} GMAIL {- dot -} COM
Date: 2008-03-14

Dear all,

Sorry for this off-topic question.

I am studying a peripheral membrane protein (13kDa) by liquid state
NMR, which is stable at pH 9.0 (50mM CHES pH9.0, 150mM NaCl and 1mM
DTT). It crashes when I lower pH of buffer. The addition of 50mM Arg +
Glu works to stabilize the protein at low pH, but interfere with the
interaction between protein and phosphatidylinositol mono-phosphate
(PtdInsP1). I've already tried some other ways to stabilize the
protein, like high salt concentration (400mM), detergents (NP40 and
Triton X-100). None of them worked.

Any input would be very helpful.

All the best,

Ju

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 14 March 2008

Previous message:
Subject: Re: exclude range within data in scala , discontinuous run in scala problem
From: hari jayaram harijay {- at -} GMAIL {- dot -} COM
Date: 2008-03-14

Next message:
Subject: Re: exclude range within data in scala , discontinuous run in scala problem
From: Phil Evans pre {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-03-15