Re: [ccp4bb] Tricks to solubilize protein

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

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CCP4bb <-- 2008 <-- March 2008 <-- 17 March 2008

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Subject: Position in protein crystallography, Novartis. Insts. for Biomedical Research - Emeryville
From: "Bussiere, Dirksen" dirksen {- dot -} bussiere {- at -} NOVARTIS {- dot -} COM
Date: 2008-03-16

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Subject: question about processing data
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-03-17

Subject: Re: Tricks to solubilize protein
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2008-03-17

Hi

The low pH and its interaction with PIP1 can be conflicting. The low
pH can modify the interaction site.
Nevertheless, one good way to study protein stability is thermal shift assay.
You can read this publication too : PubMed ID 16604423. (Rapid
determination of protein solubility and stability conditions for NMR
studies using incomplete factorial design).

Michel.

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CCP4bb <-- 2008 <-- March 2008 <-- 17 March 2008

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Subject: question about processing data
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-03-17