[ccp4bb] MSE and Refinement

Protein crystallography Programs for crystallography X-ray detectors
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CCP4bb <-- 2008 <-- March 2008 <-- 25 March 2008

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Subject: Re: ccp4i - Directories & Project Directory Error
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-03-25

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Subject: Re: Peltier Crystallisation Incubators
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-03-25

Subject: MSE and Refinement
From: "Kim, Eun Young" EunYoung {- dot -} Kim {- at -} UCSF {- dot -} EDU
Date: 2008-03-25

Hi all,

I have a question about registering Se.
Since diffraction quality of my SeMet dataset is better than a native one, I want to use a SeMet dataset (collected at a remote wavelength) for a final model while I am trying to get a decent native dataset. I tried to put MSE in some MET positions based on Fo-Fc density, but this caused an increase in R free factor after refinement (about 2 %). I'm using a Coot for model building and Refmac for refinement. If anyone has experienced to refine SeMet data, I hope to share some tips.

Thanks,
Eunyoung

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 25 March 2008

Previous message:
Subject: Re: ccp4i - Directories & Project Directory Error
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-03-25

Next message:
Subject: Re: Peltier Crystallisation Incubators
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-03-25