Re: [ccp4bb] "inclusion body"

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- April 2008 <-- 02 April 2008

Previous message:
Subject: Re: Inverse 3D profile/threading
From: Isaac Westwood isaac {- dot -} westwood {- at -} PHARM {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-04-02

Next message:
Subject: Re: "inclusion body"
From: Giles Robertson g {- dot -} robertson {- at -} MAIL {- dot -} CRYST {- dot -} BBK {- dot -} AC {- dot -} UK
Date: 2008-04-02

Subject: Re: "inclusion body"
From: Mohinder Pal mp11 {- at -} SOTON {- dot -} AC {- dot -} UK
Date: 2008-04-02

I had the similar problems with the protein expression but I tried heat
shock at 42 degree C for 25 mins before induction with IPTG and
approximately 60% of the overexpressed protein become soluble. The
explanation for this is the upregulation of heat shock proteins that may
help in the refolding of the overexpressed proteins. So it is woth to try.

Best of luck.

Mohinder

CCP4bb navigation

CCP4bb <-- 2008 <-- April 2008 <-- 02 April 2008

Previous message:
Subject: Re: Inverse 3D profile/threading
From: Isaac Westwood isaac {- dot -} westwood {- at -} PHARM {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-04-02

Next message:
Subject: Re: "inclusion body"
From: Giles Robertson g {- dot -} robertson {- at -} MAIL {- dot -} CRYST {- dot -} BBK {- dot -} AC {- dot -} UK
Date: 2008-04-02