Re: [ccp4bb] Co-expression plasmids

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Previous message:
Subject: Re: Co-expression plasmids
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-04-02

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Subject: Re: Co-expression plasmids
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-04-02

Subject: Re: Co-expression plasmids
From: Raji Edayathumangalam redayath {- at -} MAIL {- dot -} ROCKEFELLER {- dot -} EDU
Date: 2008-04-02

I thought only I was having trouble with cloning into pETDuet1. But just to add to what someone just
said.... Yes, I had hell with trying to get one of my ~2kb fragments into pETDuet1 (5.4kb). Could be
a combination of vector size and insert size.. Who knows!

Sometimes, I do what Tasos says: Transform two plasmids into cells, either sequentially or
co-transform. I just play down the amount of each DNA to be used.

Raji

---------Included Message----------
>Date: 2-apr-2008 09:56:09 -0400
>From: "Mark J. van Raaij"
>To:
>Subject: [ccp4bb] Co-expression plasmids
>
>Dear All,
>
>Anyone have experience with the NovaGen Duet co-expression vectors? Or
>can recommend others?
>http://www.emdbiosciences.com/html/NVG/Duet_Spot.html
>
>Greetings,
>
>Mark
>
>Mark J. van Raaij
>Dpto de Bioquímica, Facultad de Farmacia
>Universidad de Santiago
>15782 Santiago de Compostela
>Spain
>http://web.usc.es/~vanraaij/
>
>
>
>
>
>
---------End of Included Message----------

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CCP4bb <-- 2008 <-- April 2008 <-- 02 April 2008

Previous message:
Subject: Re: Co-expression plasmids
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-04-02

Next message:
Subject: Re: Co-expression plasmids
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-04-02