[ccp4bb] Summary: Multiple nucleation.

Protein crystallography Programs for crystallography X-ray detectors
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CCP4bb <-- 2007 <-- April 2007 <-- 05 April 2007

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Subject: antibody refinement in REFMAC with Kabat numbering
From: John Pak john {- dot -} pak {- at -} UTORONTO {- dot -} CA
Date: 2007-04-05

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From: Klaus_Fütterer k {- dot -} futterer {- at -} BHAM {- dot -} AC {- dot -} UK
Date: 2007-04-05

Subject: Summary: Multiple nucleation.
From: Jobichen Chacko jobichenc {- at -} NUS {- dot -} EDU {- dot -} SG
Date: 2007-04-05

Hai All,
Thanks for all the good suggestions.
These are the ideas I received .
Growing crystals at different temperature, 4 C, 15C,21C and 10C.
Add glycerol or ethylene glycol - set a screen with your condition as basis, and % EG/glycerol across - from 1% to 15% in suitable increments
Find additives that produce no crystals, set up tray(s) with those, then seed with microcrystals.
Reduce protein concentration. Add gelling agents to the set-up
Also one member suggested that increasing protein concentration and reducing the PEG concentration can increase the crystal size.
Microseeding.
Cross-seeding a complete screen with microcrystals.
Crystallization in sitting drops under oil.

Jobichen Chacko.

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CCP4bb <-- 2007 <-- April 2007 <-- 05 April 2007

Previous message:
Subject: antibody refinement in REFMAC with Kabat numbering
From: John Pak john {- dot -} pak {- at -} UTORONTO {- dot -} CA
Date: 2007-04-05

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From: Klaus_Fütterer k {- dot -} futterer {- at -} BHAM {- dot -} AC {- dot -} UK
Date: 2007-04-05