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Re: [ccp4bb] question on how crystals form |
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CCP4bb navigationCCP4bb <-- 2008 <-- May 2008 <-- 05 May 2008Subject: Re: question on how crystals form From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU Date: 2008-05-05 how does your protein look on a sizing column ? Perhaps a dimer ? That would then explain the tendency to form dimers in different crystal forms, as it is a biological relevant dimer. for the eventuality that you have no clue how the sizing looks like, run one and find out. Juergen Michael Colaneri wrote: > Dear colleagues, > > I have a structure with two identical dimers per asymmetric unit. If > the dimers are identical in different crystalline environments and in > different crystal forms they should be particularly stable. > > How does such a stable dimer crystallize? Is it necessay to pre-exist > in the specific conformation in solution (even to a lesser extent than > a major monomeric form) or a very stable dimer can assemble from the > very beginning durnig crystallization? And can the same dimer form > during crystallization in different crystalline environments without > it being present in solution to any (even infinitesimal) extent? > > I would appreciate all responses. > > Thanks. > > Mike Colaneri -- Jürgen Bosch University of Washington Dept. of Biochemistry, K-426 1705 NE Pacific Street Seattle, WA 98195 Box 357742 Phone: +1-206-616-4510 FAX: +1-206-685-7002 Web: http://faculty.washington.edu/jbosch CCP4bb navigationCCP4bb <-- 2008 <-- May 2008 <-- 05 May 2008 |
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