[ccp4bb] effect of high salt on protein

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CCP4bb <-- 2007 <-- April 2007 <-- 07 April 2007

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From: Paul Emsley emsley {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-04-07

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Subject: Re: effect of high salt on protein
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-07

Subject: effect of high salt on protein
From: bputcha bputcha {- at -} UTK {- dot -} EDU
Date: 2007-04-07

Hi all,
I keep my protein at 1 M NaCl, at some stage of purification, for about 30
minutes. Protein does not
precipitate out at that stage and susequently when the salt concentration is
reduced. I would like to know
if such a high salt concentration can perturb ( irreversibly) the structure or
modify the protein. Are there
any known cases?
Thank you
Dhurjati

Dept. of Biochemistry, Cellular and Molecular Biology,
Walters Life Science, # 406,
University of Tennessee, TN, Knoxville, USA

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 07 April 2007

Previous message:
Subject: Coot-0.3
From: Paul Emsley emsley {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-04-07

Next message:
Subject: Re: effect of high salt on protein
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-07