[ccp4bb] problem of crystallization

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CCP4bb <-- 2008 <-- May 2008 <-- 13 May 2008

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Subject: Beamtime available; Deadline for applications : May 15.
From: Peter Zwart PHZwart {- at -} LBL {- dot -} GOV
Date: 2008-05-13

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Subject: Re: problem of crystallization
From: Stephen Graham stepheng {- at -} STRUBI {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-05-13

Subject: problem of crystallization
From: Jennifer Han-Chun Tsai jhc {- dot -} tsai {- at -} GMAIL {- dot -} COM
Date: 2008-05-13

Hi,

This topic is not related to CCP4. I am having problem of crystallizing
one protein. It's a pretty small protein with size around 15kDa. I have
stock concentration around 100mg/mL. Crystallization plates I set up are
with concentration of 10mg/mL, 30mg/mL and 50mg/mL. All the plates had been
set up at least one week. Only around 5 wells per plate or less formed
precipitation. The rest of wells are pretty clear still. Is there any
suggestion for reducing protein solubility or increasing the chance of
getting crystals?

Thanks for your time,
Jennifer

CCP4bb navigation

CCP4bb <-- 2008 <-- May 2008 <-- 13 May 2008

Previous message:
Subject: Beamtime available; Deadline for applications : May 15.
From: Peter Zwart PHZwart {- at -} LBL {- dot -} GOV
Date: 2008-05-13

Next message:
Subject: Re: problem of crystallization
From: Stephen Graham stepheng {- at -} STRUBI {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-05-13