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Re: [ccp4bb] problem of crystallization
 

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CCP4bb <-- 2008 <-- May 2008 <-- 16 May 2008
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Subject: Re: poll: cutoff for "high resolution"
From: Graeme Winter graeme {- dot -} winter {- at -} GOOGLEMAIL {- dot -} COM
Date: 2008-05-16 		Next message:
Subject: Re: problem of crystallization
From: Clemens Grimm Clemens {- dot -} Grimm {- at -} BIOZENTRUM {- dot -} UNI-WUERZBURG {- dot -} DE
Date: 2008-05-16


Subject: Re: problem of crystallization
From: Jens-Christian Navarro Poulsen jnp {- at -} KEMI {- dot -} KU {- dot -} DK
Date: 2008-05-16
Hi Jennifer



I just want to draw your attention the following paper regarding methylation
of Lysines, which reduces the solubility of their test proteins.



Walter
2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum> TS, Meier C,
Assenberg R, Au KF, Ren J, Verma A, Nettleship JE, Owens RJ, Stuart DI,
Grimes JM.


2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum> Abstract

Lysine methylation as a routine rescue strategy for protein crystallization.

Structure. 2006 Nov;14(11):1617-22.

PMID: 17098187 [PubMed - indexed for MEDLINE]



Best regards,



Jens-Christian Navarro Poulsen

Dept of Chemistry, KU





_____

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Jennifer Han-Chun Tsai
Sent: 13. maj 2008 18:17
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] problem of crystallization



Hi,



This topic is not related to CCP4. I am having problem of crystallizing
one protein. It's a pretty small protein with size around 15kDa. I have
stock concentration around 100mg/mL. Crystallization plates I set up are
with concentration of 10mg/mL, 30mg/mL and 50mg/mL. All the plates had been
set up at least one week. Only around 5 wells per plate or less formed
precipitation. The rest of wells are pretty clear still. Is there any
suggestion for reducing protein solubility or increasing the chance of
getting crystals?



Thanks for your time,

Jennifer
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