[ccp4bb] crystallisation robot

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

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From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-05-16

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From: "Ralf W {- dot -} Grosse-Kunstleve" rwgk {- at -} YAHOO {- dot -} COM
Date: 2008-05-16

Subject: crystallisation robot
From: Patrick Shaw Stewart Patrick {- at -} DOUGLAS {- dot -} CO {- dot -} UK
Date: 2008-05-16

Joe

The Oryx robots take about 8 minutes to dispense 96 wells, plus about a
minute to pick up protein etc. Evaporation is not an issue even for 100
+ 100 nl drops, because there's an evaporation shield.

This can include microseeding into screens (the D'Arcy "MMS" method) -
which we now regard as an absolutely essential facility. Many robots
can't do this because the tips get clogged.

It can also include a simple 2-d optimization grid.

Setup is very simple because you only have to put the protein (and seed
stock) into one well.

Experiments with three drops per reservoir take about 14 minutes.

Additive experiments take longer - the tip has further to go.

Patrick

http://www.douglas.co.uk/mms.htm

http://www.douglas.co.uk/products.htm

--

patrick@douglas.co.uk Douglas Instruments Ltd.

DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK

Directors: Peter Baldock, Patrick Shaw Stewart, James Smith

http://www.douglas.co.uk/

Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034

Regd. England 2177994, VAT Reg. GB 480 7371 36

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
JOE CRYSTAL
Sent: 14 April 2008 22:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

Hi,

Does anyone have information about how long it takes to set up a 96-well
tray for the crystallization robots available? Besides cost per tray
and maintenance cost, another important feature we consider is the time
for setting up a 96-well tray. It is an important factor since we are
talking about sub-microliter drops.

Best,

Joe

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CCP4bb <-- 2008 <-- May 2008 <-- 16 May 2008

Previous message:
Subject: Re: No permission to CCP4I TOP dir
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-05-16

Next message:
Subject: Re: Phaser compile errors
From: "Ralf W {- dot -} Grosse-Kunstleve" rwgk {- at -} YAHOO {- dot -} COM
Date: 2008-05-16