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Re: [ccp4bb] Unusual Difference Fourier near Methionines
 

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CCP4bb <-- 2007 <-- April 2007 <-- 13 April 2007
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Subject: Re: Unusual Difference Fourier near Methionines
From: Ethayathulla Abdulsamath ethaya_aiims {- at -} REDIFFMAIL {- dot -} COM
Date: 2007-04-13
Dear Dr. Thomas

It's not SAD/MAD data I have solved it using molrep only.

I will try alternate positions for met and see the density again.

Thanx to all for valuable suggestions

Ethayathulla


 


On Fri, 13 Apr 2007 Thomas Edwards wrote :
>
>Dear Ethayathulla ,
>
>did you solve the structure by SeMet MAD or some other way?
>Sometimes you get strange densities around the ends of methionine side
>chains (around the S/Se) when switching from SeMet data to native.
>
>Cheers
>Ed
>
>
>______________
>T.Edwards Ph.D.
>Astbury Centre for Structural Molecular Biology
>University of Leeds, Leeds, LS2 9JT
>Telephone: 0113 343 3031
>
> -----Original Message-----
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On
>Behalf Of Ethayathulla Abdulsamath
> Sent: Fri 13 Apr 2007 10:51
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] Unusual Difference Fourier near Methionines
>
>
>
>
> Dear all
>
> I am doing one structure at 2.6A resolution where I found
>unusual density near methionines. Actually three methionines come close
>together nearby and I get difference even at 5sigma cutoff. I don't
>understand nature of the density. The amino acid sequnce is same I mean
>met by chemical sequncing so there is no change amino acid residues. I
>am sending the snapshot of the difference fourier observed at
>methionines. The crystal belong to R32 system with one molecule and
>biological trimer. In snapshot two of the methionines are symmetry
>related.
>
> Details of the data collection.
> Data collected at synchrotron
>
> Overall Rsym = 6 % and I/sigma = 2.1. Completeness = 99%
>
> Can anyone suggestion what could be chemical nature for the
>difference fourier.
>
> Thanks in advance
>
> Ethayathulla
>
> ###################################################
> A.S.Ethayathulla,Ph.D.
> Department of Biophysics
> All India Institute of Medical Sciences
> Ansari Nagar
> New Delhi-110029
> India.
> ###################################################
>
>
>
>Imagine
>
>ature-home.htm/1050715198@Middle5/1152735_1146939/1151973/1?PARTNER=3&OA
>S_QUERY=null target=new>
>


###################################################
A.S.Ethayathulla,Ph.D.
Department of Biophysics
All India Institute of Medical Sciences
Ansari Nagar
New Delhi-110029
India.
###################################################
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