[ccp4bb] Hints on oil phase in the presence precipitants?

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CCP4bb <-- 2007 <-- April 2007 <-- 19 April 2007

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From: Roger Rowlett RRowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2007-04-19

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Subject: Hints on oil phase in the presence precipitants?
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2007-04-19

Hello,

I've got a protein that forms oil phase in almost all conditions in the
screen. No xtals thus far. I was wondering if there are general hints on
dealing with such cases. Basically, I am trying to understand what
direction to go from here. E.g. decrease or increase [protein], [salt] and
general precipitant strength? High concentration of some solvent
everywhere? Detergents?

The protein is small, forms dimer via short coiled coils region and and
various prediction servers say that the rest is likely to be disordered/not
globular. In low to moderate salt without precipitants it behaves well -
single peak on gel-filtration, does not aggregate, can be concentrated to
at least 30 mg/ml.

Thanks,

Dima

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 19 April 2007

Previous message:
Subject: Re: Protein expression in Minimal media (M9)
From: Roger Rowlett RRowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2007-04-19

Next message:
Subject: Re: Summary - Stop Refmac from refining B factors?
From: "Mark A {- dot -} White" white {- at -} XRAY {- dot -} UTMB {- dot -} EDU
Date: 2007-04-19