[ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate

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CCP4bb <-- 2007 <-- April 2007 <-- 19 April 2007

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Subject: Re: How to run SIGMAA twice in a row? (fwd)
From: Huiying Li hli {- at -} CRYSTAL {- dot -} BIO {- dot -} UCI {- dot -} EDU
Date: 2007-04-19

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Subject: Re: recommendation for heavy atoms used in lithium sulfate and ammonium sulfate
From: Ronnie Berntsson r {- dot -} berntsson {- at -} RUG {- dot -} NL
Date: 2007-04-20

Subject: recommendation for heavy atoms used in lithium sulfate and ammonium sulfate
From: Tiancen Hu tony_htc {- at -} 126 {- dot -} COM
Date: 2007-04-19

Dear all,

Could any one recommend some heavy atoms used for crystals grown in 0.1M tri-sodium citrate pH 5.6, 1M Lithium sulfate and 0.5M ammonium sulfate? I read from Hampton user guide of heavy atom kit that "high salt concentrations are not the ideal medium for heavy atom reactions with macromolecules", so is there any type of heavy atom we should avoid using? We do not have any experience in preparing heavy atom derivative, so any suggestions, experience or references will be greatly appreciated.

Thanks in advance!

Tiancen Hu
Shanghai Institute of Materia Medica

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CCP4bb <-- 2007 <-- April 2007 <-- 19 April 2007

Previous message:
Subject: Re: How to run SIGMAA twice in a row? (fwd)
From: Huiying Li hli {- at -} CRYSTAL {- dot -} BIO {- dot -} UCI {- dot -} EDU
Date: 2007-04-19

Next message:
Subject: Re: recommendation for heavy atoms used in lithium sulfate and ammonium sulfate
From: Ronnie Berntsson r {- dot -} berntsson {- at -} RUG {- dot -} NL
Date: 2007-04-20