Re: [ccp4bb] Structures with pseudo-uridine?

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 27 April 2007

Previous message:
Subject: Re: Error with scala after combat
From: Phil Evans pre {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-04-27

Next message:
Subject: Re: Superposition of maps from different crystal forms
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-04-27

Subject: Re: Structures with pseudo-uridine?
From: Philippe DUMAS p {- dot -} dumas {- at -} IBMC {- dot -} U-STRASBG {- dot -} FR
Date: 2007-04-27

Yuan

All other tRNA structures, free or engaged in complex with their cognate
synthetase (as for Arnez & Steitz' structure), contain at least a
pseudoUridine. Cytoplasmic tRNAs almost invariably contain the conserved
sequence T(54)-PseudoU(55)-C(56) in the so-called TPsi-loop.
There are many such structures in the PDB and the NDB.

Philippe Dumas
IBMC-CNRS, UPR9002
15, rue Rene Descartes 67084 Strasbourg cedex
tel: +33 (0)3 88 41 70 02
p.dumas@ibmc.u-strasbg.fr

-----Message d'origine-----
De : CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]De la part de
Yuan Lin
Envoye : vendredi 27 avril 2007 03:46
A : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Structures with pseudo-uridine?

Dear All,
I solved a structure of a pseudouridylated RNA and am now starting to
write it up. Except for the ribosome, I can only find one X-ray structure
that contains pseudo-uridine (Arnez JG and Steitz TA, 1994, Biochemistry
33:7560-7567). I was wondering is there any X-ray structures that I am
missing? Also, I am looking for the references on the effect of psi on RNA
structure, and likewise have come up empty handed. Could anyone suggest some
for me? Thanks very much!

Best,
Yuan

------------------------
Yuan Lin
Graduate Student
Biochemistry and Molecular Biology
Johns Hopkins University
Rm. W8702 BSPH
615 N. Wolfe St.
Baltimore, MD 21205
Ph. 443-287-4988

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 27 April 2007

Previous message:
Subject: Re: Error with scala after combat
From: Phil Evans pre {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-04-27

Next message:
Subject: Re: Superposition of maps from different crystal forms
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-04-27