Re: [ccp4bb] Refmac and B factors

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2007 <-- May 2007 <-- 09 May 2007

Previous message:
Subject: Re: Ligand insertion and orientation...
From: James Pauff pauffjm {- at -} YAHOO {- dot -} COM
Date: 2007-05-09

Next message:
Subject: Postdoctoral Position at UCSF -- Structural Biology of Kinase-Scaffold Complexes
From: Wendell Lim lim {- at -} CMP {- dot -} UCSF {- dot -} EDU
Date: 2007-05-09

Subject: Re: Refmac and B factors
From: Peter Adrian Meyer pam52 {- at -} CORNELL {- dot -} EDU
Date: 2007-05-09

Simon,

REFI BREF OVERALL should do something close to what you're looking for (it
will refine a single B-factor for the entire model, but there shouldn't be
variation within the model).

Not sure where (or if) this is in the gui, however.

Pete

>
> Dear all,
>
> I have a structure at fairly low resolution that I am trying to refine
> with Refmac. I do not want to refine B factors so have arbitrarily set
> them all to 20 and then run refmac in the ccp4i GUI after deselecting
> the "refine temperature factors" box. However, when I look at the
> resulting pdb file my B factors vary from 2 to 90.
>
> Is Refmac just calculating my B factors or is it still refining them,
> and if the latter how can I stop it?
>
> Thanks,
>
> Simon
>

Pete Meyer
Fu Lab
BMCB grad student
Cornell University

CCP4bb navigation

CCP4bb <-- 2007 <-- May 2007 <-- 09 May 2007

Previous message:
Subject: Re: Ligand insertion and orientation...
From: James Pauff pauffjm {- at -} YAHOO {- dot -} COM
Date: 2007-05-09

Next message:
Subject: Postdoctoral Position at UCSF -- Structural Biology of Kinase-Scaffold Complexes
From: Wendell Lim lim {- at -} CMP {- dot -} UCSF {- dot -} EDU
Date: 2007-05-09