HKL2000-package: Denzo, Scalepack, XDisplayF, Strat and GUI

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

   - Programs for crystallography
       - ADSC-software
       - HKL package
       - HKL2000 package
         - XDisplayF
       - HKL2MAP
       - Pocket
   - X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

HKL2000-package general description

HKL2000-package is a set of programs significantly improved Denzo, XDisplayF, Scalepack, Strat and GUI designed for monocrystal X-ray diffraction data analysis. Most crystallographic structures deposited to RCSB Protein Data Bank (nearly 80%) solved with using the data obtained with Denzo/Scalepack programs. The HKL2000-packge is the next version of popular HKL-package.The most remarkable difference is the presence of Graphic User Interface (GUI) and Strategy program.

Key features of HKL2000-package

HKL2000 is a new extended and significantly improved version of HKL-package and therefore all features of HKL are exists and new added. • Strategy and simulation - now it is very easy to predict X-ray data collection strategy.
• 3-D processing is a new tool to handle X-ray data from crystals with very high mosaicity, or X-ray data with very fine sliced images.
• Mosaicity refinement during X-ray data integration
• It allows to process data collected with any spindle axis orientation and refine the direction of the spindle axis.
• GUI-Graphic User Interface allow to coordinate all programs.

Hardware and OS requirement for HKL2000-package

This program suite distributed as already compiled executable files and supported operational systems are:
• SGI Irix 6.5
• Linux (RedHat 9.0, RedHat Enterprise, Fedora Core, Altix SGI Enterprise)
• OSF 3.2, but this OS is not supported now and the last available version of HKL/HKL-2000 for OSF is 1.97.9 / 0.97.647d
• IBM AIX Unix

For proper operation the minimal hardware requirements are: Memory - 512 Mb, Swap Space - 1.5Gb, 16 bit or more graphics card, 1280 x 1024 screen resolution.

HKL2000-package reference

Any publications, mentioned HKL2000-package, should contain the following reference:

Z. Otwinowski and W. Minor,
Processing of X-ray Diffraction Data Collected in Oscillation Mode
Methods in Enzymology, Volume 276: Macromolecular Crystallography, part A, p.307-326, 1997,C.W. Carter, Jr. & R. M. Sweet, Eds.,