Sugar refractometer for NaCl

- Protein crystallography

Main steps:

   - Protein purification
     - Introduction
     - Step-by-Step
     - Common sense
     - Protocols
     - Charts & Tables
       - AM concentration
       - NaCl-sgar refractometer
       - Calibration of Superdex 200
       - AM table
       - UV no TRP
       - UV with TRP
     - Appendix
   - Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

Protocols and tips in protein purification or How to purify protein in one day.

NaCl vs Sugar concentration

It is possible to check the concentration of the NaCl in solution by using portable Sugar Refractometer. You only need to find the effective sugar concentration and then calculate the real concentration of NaCl using calculator below or using chart below.

Please note, that the allowed sugar concentration from this plot can vary from 0.0% to 28% and all measurements should be taken at room temperature.

NaCL concentration calculator:

NaCl, M vs % sugar chart

This is a calibration chart for NaCl refraction on Sugar refractometer.

$NaCl refraction on sugar refractometer$