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UV spectrum of the tryptophan-free protein |
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- Protein crystallographyMain steps:- Protein purification- Introduction - Step-by-Step - Common sense - Protocols - Charts & Tables - AM concentration - NaCl-sgar refractometer - Calibration of Superdex 200 - AM table - UV no TRP - UV with TRP - Appendix - Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
Protocols and tips in protein purification or How to purify protein in one day.
UV spectrum of the tryptophan-free proteinThe observed absorbance corresponds mainly to tyrosine.
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