Sugar refractometer for ammonium sulphate

- Protein crystallography

Main steps:

   - Protein purification
     - Introduction
     - Step-by-Step
     - Common sense
     - Protocols
     - Charts & Tables
       - AM concentration
       - NaCl-sgar refractometer
       - Calibration of Superdex 200
       - AM table
       - UV no TRP
       - UV with TRP
     - Appendix
   - Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

Ammonium sulphate vs Sugar concentration

It is possible to check the concentration of the Ammonium Sulphate in solution by using portable Sugar Refractometer. You only need to find the effective sugar concentration and then calculate the real concentration of Ammonium sulphate using form below or using chart below.

Please note, that the allowed sugar concentration from this plot can vary from 0.0% to 38.25%. All measurements outstanding this range can not be used without further correction for the determination of the Ammonium sulphate concentration.

AmSO₄ concentration calculator:

Ammonium sulphate, M vs % sugar chart

This is a calibration chart for Ammonium sulphate refraction on Sugar refractometer.

$Ammonium sulphate refraction on sugar refractometer$