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Ammonium sulphate table |
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Navigation: Go back to the Protein purification Navigation: Go back to the Chapter 5. Charts and tables Navigation: Go to: previous Calibration plot for Hi-load Superdex 200 column Navigation: Go to: next UV spectrum of the tryptophan-free protein Protocols and tips in protein purification or How to purify protein in one day. Ammonium sulphate tableThis table indicates the grams of solid ammonium sulphate (at 25°C) to be added to one litre of solution to produce a desired change in the presence saturation of ammonium sulphate. Saturated solution is 4.1 M and required 767 grams of salt per litre. This table was based on Green and Hughes in Methods Enzymol. 1, 67-90 (1955).How to use ammonium sulphate concentration table: Select your initial concentration of ammonium sulphate in percent from saturation from the left column, then select required final concentration of ammonium sulphate in percent from saturation from the top row. Intersection will give you and exact amount of ammonium sulphate (AmSO4) in grams for 1 litre of initial solution.
Navigation: Go back to the Protein purification Navigation: Go back to the Chapter 5. Charts and tables Navigation: Go to: previous Calibration plot for Hi-load Superdex 200 column Navigation: Go to: next UV spectrum of the tryptophan-free protein |
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