Chapter 3 - Common sense

- Protein crystallography

Main steps:

   - Protein purification
     - Introduction
     - Step-by-Step
     - Common sense
       - Common sense strategy
       - Purification protocol
       - Purification of soluble protein
       - Purification of DNA-binding protein
       - Timing of purification
     - Protocols
     - Charts & Tables
     - Appendix
   - Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

Protocols and tips in protein purification or How to purify protein in one day.

Common sense is the best guide for almost anything, especially for chemistry. Manipulations with new objects are always required new protocols, strategies and sometimes new techniques. During such investigations, you should always follow the common sense and do not do any stupid mistakes. This chapter will give you general tips and tricks which helps you to save you time and reagents during protein purification.

Chapter 3 - Common sense

Main steps:

Special:

Basic tutorials:

Xtal community:

3. "Common sense" strategy in protein purification