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Chapter 2 - General sequence of protein purification |
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- Protein crystallographyMain steps:- Protein purification- Introduction - Step-by-Step - Equipment & Reagents - Stock solutions - Chromatographic columns - Crude extract - pre Chromatographic step - Chromatographic step - Common sense - Protocols - Charts & Tables - Appendix - Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
Protocols and tips in protein purification or How to purify protein in one day. First step of any experiments is the understanding of the general sequence of all major steps. You should understand in great details what you should do and in which sequence. This is very important to prepare all necessary stock solutions, to book appropriate time to work with expensive equipments, such as centrifuges, columns etc. This plan of actions will help you to save a lot of time and be prepared to some non-standard situations. 2. General sequence of protein purification procedures
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