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Protein structure
 

Protein purification procedures. Preparation of equipment and reagents

 

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Protocols and tips in protein purification or How to purify protein in one day.

Preparation of equipment and reagents

For protein purification you need the equipment and reagents listed below:
  • Sonicator or French Press
  • Centrifuge, medium speed (30-70Kg) (J-20, Avanti J-25), Appropriate centrifugation tubes
  • Chromatographic system comprising of, as a minimum, pump and fraction collector (normally system also includes UV monitor and recorder)
  • Gradient mixer
  • Spare pump or second chromatographic system
  • Chromatographic columns packed with different types of matrixes
  • Spectrophotometer UV/VIS (190-800nm) or VIS (340-800nm)
  • Bio-Rad Protein Assay Reagent and Plastic cuvettes 1.6 ml
  • Stock solutions of buffers components
  • Concentrators (VivaSpin 20 and 6, microcone) and low speed refrigerated centrifuge
  • Filter Device such as Filter Holder or Stericup Filter Unit.
  • Apparatus and solutions for SDS-PAGE
  • Refractometer (a pocket one for sucrose is fine)
  • Pippetors, tips, eppendorf tubes, tubes for fraction collector


Navigation: Go back to the Protein purification
Navigation: Go back to the Chapter 2. General sequence of protein purification procedures
Navigation: Go to: previous Introduction
Navigation: Go to: next Stock solutions preparation


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